[关键词]
[摘要]
本文选用低值鱼蛋白进行酰化改性,研究其改性程度与酶解特性(酶解物氨态氮得率,TCA可溶性肽得率及肽分子量分布)间的关系。结果表明:酰化鱼蛋白导致氨态氮得率下降,但有利于TCA可溶性氮得率的提高。最终得到60 ℃/30 min热处理后0.3 g SA/g Pro改性的鱼蛋白酶解10 h,TCA可溶性肽得率最高达38%。
[Key word]
[Abstract]
Low-cost fish protein was acylated, and then the effect of the acylation degree on the enzymatic hydrolysis of the acylated fish protein, including yield of amino acids and TCA-soluble peptide, and molecular weight distribution of peptide, was studied in this paper. Results showed that acylation significantly reduced the yield of amino acids, but improved the TCA-soluble peptides in the hydrolysates of the fish protein. For the acylation of fish protein, the best pre-incubation temperature and time and the dosage of the acylating reagent SA were 60 ℃, 30 min, and 0.3 g/g Pro, respectively, under which the highest yield of TCA soluble peptide reached 38% after hydrolyzing the fish protein for 10 h.
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[基金项目]
广东省自然科学基金资助项目(031348);广东省农业重大攻关项目(A20302);广东省科技计划项目(2004A20302003)