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[摘要]
以副溶血性弧菌抗原免疫新西兰大白兔获得特异性抗体,利用此抗体,进行间接竞争ELISA法实验,确定了抗原抗体最佳反应浓度和一抗稀释度分别为107 cfu/mL和1:4000,酶标二抗IgG-HRP最佳工作稀释度为1:1000。以此方法分别对人工染菌水产品及实际水产品进行检测,检测下限为104 cfu/mL,检测时间为8 h;而经8 h增菌后,其检测下限为103 cfu/mL。此法与常规方法检测结果完全一致,具有很高的实际应用价值。
[Key word]
[Abstract]
The specific antibody was achieved by immune New Zealand white rabbit with V. parahaemolyticus antigen. Using this antibody, a indirect ELISA method for the detection of V. parahaemolyticus was developed with the best concentrations of the antibody, antigen and second antibody marked with HRP being of 107 cfu/mL, 1:4000, and 1:1000, respectively. The method can be used for the detection of V. parahaemolyticus in seafood with the detection limit and time of 104 cfu/mL and 8 h, respectively. However, the detection limit increased to 103 cfu/mL after an 8 h of bacteria multiplication. This rapid method had similar results with the conventional method, showing high practical application value.
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