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[摘要]
研究培养基的成分及添加量对大肠杆菌产生抗癌药物—L-天冬酰胺酶的影响。通过对大肠杆菌培养基中碳源、氮源以及微量元素的讨论,得知该菌株以蔗糖为碳源,添加量为0.7%,以0.5%牛肉膏+1.0%蛋白胨为氮源,添加0.01%的硫酸亚铁为微量元素,所产L-天冬酰胺酶的比酶活达到352.54 U/g,相比基本培养基培养提高了31%。
[Key word]
[Abstract]
The effects of the components of culture medium on the production of L-asparaginase, an anti-cancer drug fermented by E.coli, were studied. Results showed that the highest specific activity of L-asparaginase (352.54 U/g) was achieved under such conditions: 0.7% of sucrose as carbon source, 0.5% of beef extract and 1.0% of peptone as nitrogen source and 0.01% of FeSO4 as trace element. The yield of L-asparaginase was 31% higher than that by basic medium fermentation.
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