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[摘要]
运用METATOOL软件对枯草芽孢杆菌(Bacillus subtillis)TZM1012由葡萄糖生物合成胞苷的代谢途径进行分析,以确定胞苷合成的最佳途径和最大理论产率。结果表明其最大理论产率为0.28,5-磷酸核糖焦磷酸与草酰乙酸是胞苷合成途径的关键节点,发酵中后期低溶氧控制是胞苷合成的重要前提,溶氧控制在5%~10%时胞苷产量可提高15.8%。结论是以途径分析为指导,改变外界环境因子,胞苷的产量可得到显著的提高。
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[Abstract]
METATOOL soft ware was applied to analyze the cytidine biosynthesis pathway in Bacillus subtillis TZM1012.The results was as follows: PRPP and oxaloacetic acid were found to be the key nodes in cytidine biosynthesis pathway and low dissolved oxygen concentration in the medium-term and last periods of fermentation was an important precondition of cytidine biosynthesis. The highest theoretical yield of cytidine was 0.28 and cytidine yield could increased by 15.8% if the dissolved oxygen concentration in fermentation broth was of 5%~10%. According to the analysis of metabolic pathway of cytidine biosynthesis, the yield of cytidine can be greatly improved by changing the environmental factors.
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