[关键词]
[摘要]
研究了大孔树脂纯化灰毡毛忍冬绿原酸的性能。结果表明大孔树脂NKA-9可用于灰毡毛忍冬中绿原酸粗提物的纯化,其最佳工艺为:洗脱液为45%乙醇,样液浓度为20%,样液pH值为5,上柱流速为0.5 BV/h,洗脱流速为1 BV/h,洗脱液用量为4 BV。
[Key word]
[Abstract]
The purification of chlorogenic acid from Lonicera macranthodes Hand-Mazz by macro porous resin was studied. HPLC analysis showed that the purity and yield of achieved chlorogenic acid were 75.68% and 82.38%, respectively. The NKA-9 resin showed good properties of sorption and desorption.
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[基金项目]
湖南省科技厅基金项目(B-138).