木糖醇是一种功能性甜味剂。本文研究了不同发酵温度、pH、装液量、接种量、转速等对尖孢镰刀菌（Fusarium xysporum）发酵木糖的影响。结果表明：F. xysporum有较强的转化木糖的能力。在温度28~30 ℃，初始pH 5.0~6.0，装液量50%，接种量10%，转速160 r/min的优化条件下，在含木糖10 g/L培养液中添加1%甲醇时，木糖转化为木糖醇的产率为3.81%，表明用丝状真菌发酵产木糖醇是一条新的途径。

Fermentation conditions for xylitol production by Fusarium xysporum were optimized. The results showed that the optimal temperature, pH, liquid volume, inoculum size, shaking speed and methanol content in 10 g/L xylose-containing medium were 28~30 ℃, 5.0~6.0, 50%, 10%, 160 r/min and 1%, respectively, under which the conversion rate of xylose was 3.81%. This research provided a novel route for xylitol production by Fusarium xysporum.