目的：研究软骨多糖治愈的S180荷瘤小鼠的免疫功能及灭活S180细胞对小鼠免疫功能的影响，分析其免疫机制。方法：用MTT法测定软骨多糖治愈的S180腹水瘤小鼠的血清、脾细胞及胸腺细胞对S180细胞和MCF-7乳腺癌细胞的生长影响，并以灭活的S180细胞对健康小鼠免疫，观察其对小鼠免疫功能的影响。结果：软骨多糖治愈的S180荷瘤小鼠的血清、脾细胞及胸腺细胞对肿瘤生长均有一定抑制效果。其中在不含胎牛血清的RPMI 1640培养基培养下，治愈小鼠10%的血清对S180细胞和MCF-7乳腺癌细胞的抑制率分别为64.09%、73.48%，而其20%血清对两种肿瘤细胞的抑制率则分别为88.70%、96.80%。结论：软骨多糖治愈的S180荷瘤小鼠对肿瘤有一定的免疫能力。

Objective: To study the immune function of the S180 tumor-bearing mice cured by cartilage polysaccharide, the effects of virucidal S180 tumor on immune function of the mice and the mechanisms. Methods: The influences of the blood serum, spleen cells and thymucytes of the S180 ascites tumor-bearing mice cured by cartilage polysaccharide on the S180 tumor and MCF-7 breast carcinoma cells were measured using the MTT method. Virucidal S180 tumor cells were injected into healthy mice and their effect on immune functions of the mice were investigated. Results: The blood serum, spleen cells and thymucytes of the S180 tumor-bearing mice cured by cartilage polysaccharide had definite inhibitory effects on tumor growth. When cultured in the RPMI 1640 culture-medium, the inhibition rates of 10% blood serum of the S180 tumor-bearing mice cured by cartilage polysaccharide reached 64.09 %, 73.48 %, respectively, while those of 20% blood serum of the mice were 88.70 % and 96.80 %, respectively. Conclusion: The S180 tumor-bearing mice cured by cartilage polysaccharide had definite immune functions on tumors.

国家自然科学基金项目（20576101）