目的：研究紫锥菊商业提取物中有效成分菊苣酸的含量，为紫锥菊提取物的进一步应用提供依据。方法：HPLC-PDA法，C18柱，柱温35 ℃。流动相为色谱纯乙腈和0.1%的磷酸溶液，采用梯度洗脱，流速1.5 mL/min，检测波长为330 nm。结果：各组分分离较好，菊苣酸在0.456~2.28 μg线性关系良好（r=0.9996）。平均加样回收率为98.18%，RSD为1.12%。结论：该法定性定量准确、可靠、重现性好，可以用于紫锥菊提取物中有效成分菊苣酸的质量评价。

Objective To establish a method for the determination of cichoric acid, an active component in commercial extracts of Echinacea purpurea, by HPLC-PDA. and provide reference for the further use of Echinacea purpurea extracts. Method The samples were analyzed by HPLC-PDA on a C18 column with a PDA at 330 nm and the temperature at 35 ℃. The mobile phase was a mixture of acetonitrile and 0.1% phosphoric acid at a flow rate of 1.5 mL/min. Results The standard curve was linear in the cichoric acid concentration range of 0.456~2.28 μg (r=0.9996). The average recovery was 98.18% (RSD=1.12%). Conclusion The method was rapid, sensitive and had good reproducibility, which could be used for quality assessment of cichoric acid in commercial extracts of Echinacea purpurea.