探讨软骨多糖（CA）在体内抑制H22肝癌细胞生长的作用及其机制。以H22细胞腹水瘤模型为研究对象，设对照组、CA组两组，分别考察CA对各组小鼠的生活状态的影响。并且应用苏木素伊红（HE）染色、原位末端脱氧核苷酸转移酶标记法（TUNEL）、流式细胞术（FCM）及免疫荧光法对肿瘤细胞的生长和凋亡进行研究。CA可以在一定程度上促进H22细胞的凋亡，抑制肿瘤细胞的生长，延长小鼠的生存时间。并且，随着用药时间的延长，CA组的P21表达增强，而CyclinD1表达水平降低。软骨多糖有促进肿瘤细胞凋亡的作用，是一种新型的抗癌药物，具有广泛的应用前景。

The effect and mechanism of inhibition of H22 Hepatocarcinoma cell growth by cartilage polysaccharides (CA) were studied. Transplanted H22 hepatocarcinoma was used as the model and the mice were randomly divided into 2 group, the control group and CA group. The growth and apoptosis of the tumor cell were investigated by HE Staining, terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL), flow cytometry (FCM) and immunofluorescent staining. Results showed that CA could induce the apoptosis of the H22 cell, inhibit the growth of tumor and prolong the life span time of the examined mice. Besides, increasing the time after taking medicine could enhance the expression of P21 protein in the CA group and inhibit the expression of Cyclin D1. As a new anti-cancer agent, CA had wide application prospects.

国家自然科学基金项目（20776113）