[关键词]
[摘要]
本文探索了利用碱浸提和酶法从淡水鱼鳞中提取糖缀合物的技术,通过考察NaOH浓度、料液比、提取时间对糖缀合物提取效率的影响,确定了其提取的最佳条件为:0.3 mol/L NaOH溶液、料液比为1:5、室温提取18 h。对提取物初步分析后,进行体外抗氧化活性研究,结果表明鱼鳞糖缀合物对DPPH•、O2.-、•OH有较强的清除能力,同时也有较好的还原能力和抗脂质过氧化能力。
[Key word]
[Abstract]
The alkaline and enzymatic extractions of a glycoconjugate from fish scale were researched. The best extraction reagent, the concentration of the extraction reagent, the solid- fluid ratio and the extracting time were found to be NaOH solution, 0.3 mol/L , 1:5 and 18 h, respectively. Evaluation of the antioxidatnt activity of the achieved glycoconjugate showed that the glycoconjugate could efficiently scavenge DPPH•, O2.- and •OH . Besides, it had high reducing capacity and strong ability to prevent lipid peroxidation.
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[基金项目]
天津市科技大学引进人才科研启动基金资助(20060422)