采用乙醇沉淀，在抗坏血酸和还原铁粉、50 ℃水浴的条件下，富血红素多肽酶解液及其超滤分离组分（透过液和截留液）以2:1比率（v/m）分别与FeCl2反应1 h得总铁含量为127.34 mg/g，128.70 mg/g和47.83 mg/g的多肽螯合铁。红外光谱分析表明：多肽螯合铁形成新的－C≡N特征吸收峰，证明了多肽与铁形成新的化学结构。脂肪储藏实验表明：在促进脂肪过氧化值升高方面，富血红素多肽螯合铁比富血红素多肽酶解液和透过液要强，但比FeCl2弱。

The enzymatic hydrolysate, which was rich in ferrous peptides, were reacted with FeCl2 at a ratio of 2:1 in a water bath at 50 ℃ for 1 h in the presence of antioxidants, then the mixtures were centrifuged at 15000 r/min for 10 min and the supernatant was precipitated by ethanol. The precipitate was washed several times by ethanol, followed by a vacuum dry. Ferrous peptides were obtained with content of total iron being of 127.34 mg/g (heme-enriched peptide), 128.70 mg/g (permeate peptide) and 47.83 mg/g (retentate peptide), respectively. The structure of the new compound was identified by Infrared spectrometry. It was also found that oxidation stability of ferrous heme-enriched peptide was higher than that of FeCl2 but lower than that of heme-enriched peptide or permeate peptide.

广东省科技计划项目“粤港关键领域重点突破招标项目”（2005A20303003）；广东省自然科学基金项目（031348）