本文研究了摇瓶液体培养的不同培养条件对蛹虫草液体培养生产虫草素的影响。实验结果表明，蛹虫草液体培养生产虫草素的最宜条件为：培养温度25 ℃，转速150 r/min，初始pH 5.0。在此条件下，经摇瓶培养，获得的生物量和虫草素产量分别为16.20 g/L和264.00 mg/L。

The influences of cultivation conditions on the cordycepin production via submerged cultivation of Cordyceps militaris in the shake flask, were investigated by single factor and orthogonal experiments. The results indicated that the optimal temperature, shaking speed and initial pH value for cordycepin production were 25 ℃, 150 r/min and 5.0, respectively, under which 16.20 g/L of biomass and 264.00 mg/L of cordycepin were achieved.