本文针对脂肪酶Lecitase®Ultra的特殊性（同时具有脂肪酶活性和磷脂酶活性），采用无油磷脂为底物的酸碱滴定法测定Lecitase®Ultra的磷脂酶酶活，探讨了测定中的几个关键因素对酶活测定值的影响，建立了一套快捷可靠的磷脂酶酶活测定方法。

A method for measurement of the phospholipase A1 activity by acid-base titration with deoiled phospholipid as substrate was adopted in this paper, according to the characteristic of lipase Lecitase® Ultra which had both high lipase and phospholipase activity. The effect of some key factors on the measurement of enzymatic activity was evaluated, and a rapid and reliable method for measuring the phospholipase A1 activity of Lecitase® Ultra was established.