研究高效液相色谱法测定虫草发酵菌丝粉中腺苷含量的方法，对检测波长、流动相比例、图谱的特性、回收率及样品的超声波前处理等进行了讨论。实验表明：在反相键合的ODS Hypersil（5 μm，125×4 mm）色谱柱上，以0.06 mol/L磷酸二氢钾-甲醇-四氢呋喃（150:10:1.5, v/v/v）为流动相，检测波长UV260 nm，平均回收率为99.11 %。该方法灵敏度高、选择性好，样品前处理方法简单快速，回收率符合试验要求。

The method for determination of adenosine in zymolytic powder of aweto was studied. The detecting wavelength, proportion of mobile phase, chromatography mapping character, adenosine recovery ratio and the ultrasonic pretreatment were discussed. Reslutes showed that, using a reverse-phase bonded ODS Hypersil column (5 μm, 125×4 mm), the most suitable mobile phase and detecting wavelength were 0.06 mol/L KH2PO4- methanol-tetrahydrofuran (150:10:1.5) and 260 nm, respectively, under which the average recovery was 99.11%. The method was very sensitive and selective and the pretreatment method was simple and fast.