采用分光光度法测定鱼皮（鱿鱼皮和狗鲑鱼皮）中的羟脯氨酸（Hyp）含量。结果表明：两种鱼皮中羟脯氨酸的含量分别为0.823%和2.247%，平均回收率分别为98.47%和98.39%，变异系数分别为1.65%和0.64%。该法重复性好，可信度高，可作为判定鱼皮中胶原蛋白含量的一个定量指标。

The hydroxyproline (Hyp) content in two fish skins, squid skin and chum salmon skin, was determined by the spectrophotometric method and was shown to be 0.823% and 2.247%, respectively. The average recovery rates for the two skins were 98.47% and 98.39%, respectively, and the CV of Hyp from the two skins were 1.65% and 0.64%, respectively. This method can be used as a reliable method for the quantitative determination of the collagen in fish skins.

大连市优秀青年基金项目（2005J22JH049）