用固相微萃取（SPME）及溶剂（乙醇、乙酸乙脂）提取法对蜂胶挥发性成分进行提取，用GC毛细管柱进行分离，归一化法测其相对含量，气相色谱-质谱联用法对蜂胶中不同挥发性成分进行测定。结果SPME技术对蜂胶中萜烯类物质有简单快速，提取效率高、溶剂消耗低及一定的浓缩富集作用等优点，蜂胶含主要挥发性成分28种，可检测到十几种萜烯类及醇类物质。通过SPME-GC/MS联用技术对蜂胶挥发性成分的研究，为蜂胶的开发利用及指纹图谱的建立提供实验依据。

Propolis is a resinous substance collected by honeybees from various plant sources. SPME and solvent extraction methods were used to extract the volatile components from Chinese propolis. The major volatile constituents in Chinese propolis were identified by gas chromatography with a mass spectrometric detector and each component was quantitatively analyzed. SPME has the advantage of rapidly extracting and concentrating terpenes of analytical interest from propolis. Twenty-eight major compounds were identified, in which there are many terpenes compounds. This study can provide references for the further study of propolis.

广州市科技计划项目，蜂胶质控平台、指纹研究(2005Z-E0101)