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[摘要]
以麦芽糖和蔗糖为葡萄糖基供体检测了67株包括丝状真菌、酵母菌和细菌在内的微生物催化合成熊果苷的能力,采用高效液相色谱法测定转化液中熊果苷的含量,利用质谱法和比旋光度测定法分析产物的分子量和构型。结果表明催化合成熊果苷的能力在微生物中是很普遍的,被检测的微生物中70%以上能不同程度催化合成熊果苷,大多数微生物合成的为β-熊果苷,少数微生物合成的为α-熊果苷。
[Key word]
[Abstract]
The catalytic capability of 67 Strains of mold, yeast and bacteria, for the biosynthesis of arbutin, were investigated. The molecular weight and configuration of arbutin were determined by HPLC, Electron Ionization-Mass Spectrometry and polarimetry. The results indicated that there were more than 70% of microorganisms which could produce enzyme for catalyzing the biosynthesis of arbutin and the superior product were β-arbutin.
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