目的：建立薄层扫描法测定微生物发酵转化液中熊果苷含量的方法。方法：微生物发酵转化液经过滤浓缩制备，以乙酸乙酯-乙酸-水（v乙酸乙酯:v乙酸:v水=3.1:1:1）为展开剂，检测波长570 nm。结果：熊果苷在2~6 μg范围内线性良好，相关系数ｒ=0.9976。结论：本法快捷、简便、准确，可用于微生物发酵转化液中熊果苷含量的测定。

Objective: To establish a method for determination of the content of arbutin in microbe conversion solution by Thin-Layer chromatography. Methods: The microbe conversion solution was filtrated and condensed. The developing solvent was a mixture of ethyl acetate, acetic acid and water (3.1:1:1, v/v/v). The detection wavelength was set at 570 nm. Results: The calibration curve was Linear in the range of 2 μg~6 μg with r value of 0.9976. Conclusions: The method was quick, simple and accurate for the determination of content of arbutin.