本文比较了酶浓度、菌龄、渗透压稳定剂以及酶解温度和时间等因素对金针菇原生质体得率的影响。试验结果表明：液体培养4 d，固体培养10 d的金针菇菌丝，以0.5 mol/L KCl作渗透压稳定剂，加入1%纤维素酶和1%溶菌酶在25 ℃下酶解1.5 h，分离原生质体效果最佳，原生质体产量可达(5~7)×107 个/ml以上。

The effects of some factors on the production of protoplasts of flammulina velutipes, such as enzyme dosage, culture age, osmotic stabilizer, hydrolytic temperature and time, were investigated and the optimum conditions for preparation of protoplasts were also investigated. After 4-day liquid culture and 10-day solid culture, the Flammulina velutipes Mycelia was hydrolyzed by 1% cellulase and 1% lysozyme for 1.5h at 25℃ with 0.5M KCl as osmotic stabilizer. The maximum yield was achieved to be above (05~7)×107/ml .