以水解液中α-氨基态氮含量为指标，选用木瓜蛋白酶、碱性蛋白酶、中性蛋白酶和动物蛋白水解复合酶对吉尾鱼进行单酶水解，确定中性蛋白酶为水解用酶。通过正交实验确定的中性蛋白酶水解条件为肉:水=1:2、酶浓度1600 IU/g原料、温度60℃、水解时间5h。验证实验表明，水解液中α-氨基态氮含量为1.764 g/100g，水解度为62.2%。水解液经喷雾干燥，得到淡黄色，带海鲜味的粉末。

The enzymatic hydrolysis of Trachurus japonicus protein by papain, neutral proteinase ,alkali proteinase and composite enzyme were studied and the neutral proteinase is shown to be the most suitable one. The optimal pH value, reaction temperature, enzyme dosage, reaction time and the ratio of muscle to water were 6.8, 60℃, 1600 IU/g, 5.0 hour and 1:2, respectively, under which the rate of enzymatic hydrolysis was 62.2%.