[关键词]
[摘要]
本课题成功地从自然界分离筛选出了Xy-1、Xy-5两株高产木聚糖酶菌株,并利用玉米芯为原料制备了木聚糖,对木聚糖的制备及酶解条件进行了探讨。结果表明:木聚糖最佳的酶解条件为pH 5.0、温度50 ℃、酶解时间18 h;用10% NaOH 85 ℃下恒温浸提4 h,木聚糖的提取率最高。
[Key word]
[Abstract]
In this project Xy-1 and Xy-5 were successfully selected form the nature with high xylanase-producing activity. The xylan was prepared using corncob as raw material and the conditions of enzymatic hydrolysis and extraction of xylan were also discussed. The results showed that the best reaction temperature, pH value, and reaction time for the enzymatic hydrolysis of xylan were 50 ℃, 5.0 and 48 h, respectively. For its extraction, the most suitable NaOH content, temperature and extraction time were 10%, 85 ℃ and 4h, respectively.
[中图分类号]
[基金项目]