纤维堆囊菌产生的次级代谢产物埃博霉素是受医学界广泛关注的抗癌良药，由于其较低的产量阻碍了其工业化的发展。本文采用纤维堆囊菌DSM11999作为出发菌株，通过NTG化学诱变的方法，诱变浓度300 μg/mL，处理时间30 min，来筛选优良菌种，最终得到两株埃博霉素A的产量分别为17.3 mg/L和17.4 mg/L优良菌株。

Epothilone is a secondary metabolite of Sorangium cellulosum and a kind of highly effective anti-cancer medicine. However, its yield is very low, which blocks its industrial fermentation production. In this paper, several mutant strains were obtained by NTG mutation with NTG concentration and mutation time being of 300 μg/mL and 30 min, respectively. Finally two high epothilone A-producing strains were obtained and their yields reached 17.30 mg/L and 17.40 mg/L.

广东省自然科学基金资助项目（031357）