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[摘要]
本文研究了以茶末和废枝叶为原料提取茶多酚的工艺。工艺流程为:(1)原料经微沸水浸提后加入碳酸氢铵调pH至中偏酸性后用硫酸锌沉淀纯化;(2)然后用硫酸解析溶解沉淀,加入乙酸乙酯进行萃取,分相后,水相(硫酸锌溶液)循环供沉淀纯化用,有机相(乙酸乙酯+茶多酚)真空分离、干燥制取高纯茶多酚,萃取剂乙酸乙酯回收循环使用;茶末、废茶枝叶和碳酸氢铵、硫酸进行茶多酚的浸提和纯化后,以有机多元复肥形式回收综合利用。茶多酚的综合提取率大于13%,质量达到GTP-l(大于95%)要求。
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[Abstract]
In this paper, isolation and purification of tea polyphenols from tea dust, waste tea branches and leaves were systematically studied. Firstly, several key factors in water extraction of tea polyphenols was investigated and the most suitable ratio of tea leaves to water (g/ml), extraction temperature and extraction times were 1: 25, 95℃, 3 times (each time for 30min), respectively. Then, various precipitators were tried to purify the achieved crude tea polyphenols and ZnSO4 was shown to be the best one. Using Zn2+ as precipitator, the optimal precipitator additive dosage and pH value (regulated with NH4HCO3) were 5~6% and 6.4-6.8, respectively. Furthermore, the purified tea polyphenols was dissolved in sulfuric acid and extracted with ethyl acetate. The collected solution was evaporated and dried to give the final product. The total extraction ratio of tea polyphenols was above 13%.
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