研究了以海藻酸钠为载体，用包埋法制备固定化脂肪酶的条件，将一定量的海藻酸钠用蒸馏水加热溶解，将500mg脂肪酶溶解在pH=6的缓冲溶液中，然后两者混合均匀，用注射器将海藻酸钠酶液逐滴到1%的无菌CaCl2溶液中，搅拌条件下室温固定1h，过滤，洗涤，干燥，得球状固定化脂肪酶。试验表明，此固定化酶具有良好的耐热性能，在60℃下加热固定化脂肪酶和游离脂肪酶1.5h，固定化酶活力仅损失30%，而游离脂肪酶的酶活只为原来的18%。固定化脂肪酶还有良好的可操作性，连续使用反应10次，固定化脂肪酶的酶活依然为初始酶活的95%。

The conditions of the immobilized lipase using sodium alginate as carrier were studied. First, sodium alginate solution (dissolved with hot distilled water) and 500 mg lipase solution (dissolved with buffer solution (pH=6)) were mixed and the obtained mixture was dropped into 1% aseptic CaCl2 solution using an injector. After immobilized for 1h with shaking, filtration, washing and drying, the round immobilized lipase was finally obtained with good thermal stability. The residue activities of the immobilized lipase and free lipase after incubated at 60℃ for 1.5h were 70% and 18%, respectively. Moreover, the immobilized lipase had good operational stability, which could be repeatedly used for 10 times and the relative activity of the lipase was as high as 95%.