为实现食品中沙门氏菌的简便和快速现场检测，本研究采用FTA膜（Flinders technology associates，FTA）结合跨越式滚环等温扩增（Saltatory rolling circle amplification，SRCA）方法（FTA-SRCA）建立一种新型的沙门氏菌检测方法。利用FTA膜快速提取模板DNA，根据沙门氏菌的invA基因设计及筛选引物，建立FTA-SRCA反应体系。扩增反应在能够实现集约化检测的凹孔板中进行，反应结束后添加荧光染料观察结果。确定了该方法的特异性、灵敏度和人工污染样品的检出限，并对60个实际样品进行检测，评估其敏感性、特异性和符合率。结果表明：检测的17株沙门氏菌均为阳性结果，29株非沙门氏菌均为阴性结果，特异性良好。FTA-SRCA方法的灵敏度为6.81×100 CFU/mL，比PCR方法高100倍，比SRCA方法高10倍。对于人工污染的牛奶样品检测，FTA-SRCA方法的检出限为3.22×100 CFU/mL，比PCR方法低1000倍，比SRCA方法低10倍。检测实际样品的敏感性、特异性和符合率分别为100.00%，94.64%，95.00%。本研究建立的FTA-SRCA方法具有操作简便快速、成本低廉、特异性强、灵敏度高、检出限低等优点，可用于食品中沙门氏菌的大批量集约化快速现场检测。

In order to achieve the simple and rapid on-site detection of Salmonella in food, saltatory rolling circle amplification (SRCA) method combined with FTA card (FTA-SRCA) was used to establish a new detection method for Salmonella. The template DNA was extracted by FTA card quickly. According to the specific invA gene of Salmonella, primers were designed and screened. Based on the above, the FTA-SRCA reaction system was established. The reaction was amplificated in the well plate which can achieve intensification. After the reaction, fluorescent dyes were added to observe the results. Its specificity, sensitivity and detection limit of artificial contaminated samples were determined. Furthermore, 60 actual samples were tested to evaluate relative sensitivity, specificity and accuracy. The results indicated that the 17 strains of Salmonella tested were all positive, and the 29 strains of non-Salmonella were negative. Therefore, the method had good specificity. The sensitivity of the FTA-SRCA was 6.81×100 CFU/mL, which was 100 times that of the PCR method and 10 times that of the SRCA method For the detection of artificially contaminated milk samples, the detection limit of the FTA-SRCA was 3.22×100 CFU/mL, which was 1000 times that of the PCR method and 10 times that of the SRCA method. The sensitivity, specificity and accuracy of FTA-SRCA were 100.00%, 94.64% and 95.00%, respectively. The FTA-SRCA method established in this study had the advantages of simple and rapid operation, low cost, strong specificity, high sensitivity, and low detection limit, which can be used for the intensive and rapid on-site detection of Salmonella in food.

国家自然科学基金项目(31371772)；河北省自然科学基金重点项目(C2019204342)；河北省重点研发计划项目(18275501D)