为探究多廿烷醇在胃肠道系统及整体动物的吸收和代谢情况，本文分别利用单相静态胃肠模拟系统、大鼠在体肠循环灌流及整体动物进行了一系列研究。研究结果显示：多廿烷醇能在单相静态胃肠模拟系统中被代谢成C12:0、C16:0和C18:0三种饱和脂肪酸，人工肠液比人工胃液多降解42%的多廿烷醇并转化成饱和脂肪酸；在大鼠在体肠循环灌流实验中，多廿烷醇Papp值为7.02×10-2 cm/min，其易被大鼠肠道吸收，且在肠道中可被代谢成饱和脂肪酸和一定量的不饱和脂肪酸；在小鼠整体动物实验中，100 mg/kg多廿烷醇灌胃1、4、12 h后检测烷醇和脂肪酸，发现多廿烷醇能以原型进入机体，也能通过肠道代谢成脂肪酸进入机体，影响血清脂肪酸水平，使动物血清某些不饱和脂肪酸水平增加。本实验揭示，多廿烷醇可在胃肠道代谢，并以烷醇原型或者代谢产物的形式进入机体，影响了机体脂肪酸水平，这可能是其发挥血脂调节功能的物质基础。

To investigate the gastrointestinal absorption and metabolite analysis of policosanol in vitro and in vivo, single-phase static gastrointestinal simulation system, in situ circulating intestinal perfusion in rats and integral animal experiments were conducted. The results showed that policosanol could be metabolized into three saturated fatty acids including C12:0, C16:0 and C18:0 in the single-phase static gastrointestinal simulation system. Compared with artificial gastric juice, artificial intestinal juice degraded 42% more policosanol, which was converted to the saturated fatty acid. In intestinal system of rat, policosanol could be absorbed easily by the intestinal tract of rats, and the Papp value of policosanol was 7.02×10-2 cm/min. Moreover, policosanol were able to be brokn down into fatty acids and unsaturated fatty acids in the intestinal tract. In the integral animal experiments, at 1, 4, 12 hours after oral gavage of 100 mg/kg policosanol, the alkanol and its metabolites fatty acid could be identified in the serum of mice, which may contribute to regulate serum fatty acid levels and increase the serum level of some unsaturated fatty acids. In summary, our results revealed that policosanol can be metabolized and absorbed by the gastrointestinal tract and can also be detected in the animal body in the form of prototypes or metabolites, affecting the level of fatty acids in the body, which may be the basis for its lipid regulation function.

广州市科技计划项目(201707010357)；广东省科学院发展专项资金项目(2020GDASYL-20200103069)