佐夫色绿藻具有多种营养生长方式且可以在诱导条件下合成虾青素，是一种藻源天然虾青素生产的新型替代微藻。本研究系统探讨了以醋酸钠为碳源时，醋酸钠浓度、补料方式、pH以及乙醇对色绿藻积累虾青素的影响，并在室外300 L管道光生物反应器中对上述优化的诱导条件进行放大验证。结果表明：采用2.5 g/L醋酸钠、每6 d补加2.5 g/L醋酸钠、培养基pH控制在6.5~8.3和添加2%乙醇时，虾青素的含量最高为3.71 mg/g，较优化前的虾青素的含量提高了31.71%，产量为5.37 mg/L。在室外300 L管道光生物反应器中，其最高虾青素含量和产量分别为3.44 mg/g和1.10 mg/L。本研究采用不同诱导条件强化佐夫色绿藻胞内虾青素的积累，验证了佐夫色绿藻在户外光生物反应器规模诱导培养以生产虾青素的可行性。

Chromochloris zofingiensis can grow in a variety of vegetative growth modes and synthesize astaxanthin under inductionconditions, and thus, is regarded as a promising and new alternative producer for microalgae-derived natural astaxanthin. The present studysystematically investigated the effects of sodium acetate concentration, feeding method, pH, and ethanol concentrations on the accumulation ofastaxanthin in C. zofingiensis, and the optimized conditions were then applied to an outdoor 300 L tubular photobioreactor for large-scaleverification. The results demonstrated that when the initial sodium acetate concentration was at 2.5 g/L, sodium acetate at 2.5 g/L was addedevery 6 days, medium pH was 6.5~8.3 and 2% ethanol was added, the astaxanthin content was the highest (3.71 mg/g), which increased by 31.71% compared to that achieved before optimization, and the corresponding astaxanthin yield of astaxanthin reached up to 5.37 mg/L. In the 300 L tubularphotobioreactor, the maximum astaxanthin content and yield were 3.44 mg/g and 1.10 mg/L, respectively. The present workenabled the enhancement on the accumulation of intracellular astaxanthin in C. zofingiensis using different induction conditions, and furtherverified the feasibility of cultivating C. zofingiensis in outdoor tubular photobioreactors for astaxanthin production.

广东省公益研究与能力建设项目（2016A010105001）