探究香菇多糖基于维生素D-FGF-23-Klotho轴对维生素D缺乏大鼠骨代谢的影响。选取50只SD健康大鼠，10只作为对照组，其余40只建立维生素D缺乏模型，分为模型组、维生素D3组（1000 U/kg）、低剂量香菇多糖组（150 mg/kg）和高剂量香菇多糖组（450 mg/kg）各10只。检测大鼠血清钙、磷、碱性磷酸酶水平、维生素D-FGF-23-Klotho轴因子水平、骨代谢指标水平。研究结果显示，对照组大鼠血清25（OH）VD3、1,25（OH）VD3、FGF-23、Klotho水平分别为（75.58 ng/mL、4.98 ng/mL、468.25 pg/L、62.58 pg/mL），模型组大鼠25（OH）VD3、1,25（OH）VD3、Klotho下降至（34.35 ng/mL、3.52 ng/mL、40.12 pg/mL），FGF-23水平上升至（896.35 pg/L），与对照组相比差异具有统计学意义（p<0.05）。高剂量香菇多糖组大鼠25（OH）VD3、1,25（OH）VD3、Klotho水平上升至（71.24 ng/mL、4.85 ng/mL、58.68 pg/mL），统计上高于维生素D3组、低剂量香菇多糖组，FGF-23下降至（552.23 pg/L），统计上低于维生素D3组、低剂量香菇多糖组（p<0.05）。香菇多糖通过作用于维生素D-FGF-23-Klotho轴，调控轴因子，增加维生素D的含量，改善因维生素缺乏所导致的成骨障碍，起到保护维生素D缺乏大鼠骨质的作用。

To investigate the effect of Lentinan on bone metabolism in vitamin D deficient rats via vitamin D-fibroblast growth factor-23-Klotho axis, fifty SD healthy rats were selected, with 10 as control group, and the remaining 40 as the vitamin D deficient models (which were divided into the model group, vitamin D3 group (1000 U/kg), low-dose lentinan group (150 mg/kg) and high-dose lentinan group (450 mg/kg), with 10 rats per each group). The levels of serum calcium, phosphorus, alkaline phosphatase, vitamin D-fibroblast growth factor (FGF)-23-Klotho axis factors and bone metabolism indices were measured. The results showed that the serum levels of 25 (OH) VD3, 1,25 (OH) VD3, FGF-23 and Klotho in the control group were 75.58 ng/mL, 4.98 ng/mL, 468.25 pg/L, 62.58 pg/mL, respectively, while for the model group, the 25(OH)VD3, 1,25(OH)VD3 and Klotho decreased to 34.35 ng/mL, 3.52 ng/mL and 40.12 pg/mL, respectively, and the FGF-23 level increased to 896.35 pg/L. The differences between the data of these two groups of rats were statistically significant (p<0.05). The levels of 25 (OH) VD3, 1, 25 (OH) VD3 and Klotho in the high dose lentinan group increased to 71.24 ng/mL, 4.85 ng/mL and 58.68 pg/mL, respectively, which were statistically higher than those in the vitamin D3 group and the low dose lentinan group. Whereas, the level of FGF-23 of the high dose lentinan group decreased to 552.23 pg/L, which was statistically lower than those in the vitamin D3 group and the low dose lentinan group (p<0.05). Lentinan polysaccharides acts on the vitamin D-FGF-23-Klotho axis, regulates the axis factors, and increases the vitamin D content to improve osteogenesis and protect the bones of the vitamin D deficient rats.

河南省优秀社科调研课题（SKL-2019-575）