探究赤芝富硒蛋白对肝癌HepG2细胞增殖、凋亡、周期分布、侵袭、迁移能力的影响。选取人肝癌HepG2细胞，分为空白组、药物对照组、无硒赤芝蛋白组、低、中、高浓度组，检测六组细胞生物学行为及Bcl-2、Bax、caspase3表达。结果显示，高浓度组细胞增殖率低于其他各组，凋亡率高于其他各组，G1期细胞比例为58.66%，高于其他各组。高浓度组细胞侵袭、迁移数分别为28.21、29.66，低于其他各组（p<0.05）。高浓度组细胞Bcl-2、caspase3表达分别为0.98、1.01，均低于其他各组；Bax表达为1.03，高于其他各组（p<0.05）。使用赤芝富硒蛋白进行干预，能够影响肝癌细胞增殖、凋亡，阻滞细胞周期，抑制侵袭、迁移能力，调控Bcl-2、Bax、caspase3表达，说明赤芝富硒蛋白能够调控肝癌HepG2细胞增殖、凋亡、侵袭、迁移能力，抑制肝癌HepG2细胞的发展和扩散，为肝癌的临床治疗提供一定的理论依据。

To explore the effect of Ganoderma selenium rich protein on HepG2 cell proliferation, apoptosis, cycle distribution, invasion and migration, human hepatocellular carcinoma HepG2 cells were selected and divided into blank group, drug control group, selenium-free chiroderma lucidum protein group, low, medium and high concentration groups. Biological behaviors of cells and expressions of bcl-2, Bax and caspase3 in the six groups were detected. The results showed that the cell proliferation rate of high concentration group was lower than that of other groups, and the apoptosis rate was higher than that of other groups. The number of cell invasion and migration in high concentration group was 28.21 and 29.66, respectively, lower than that in other groups (p<0.05). The expression of Bcl-2 and Caspase-3 in the high concentration group was 0.98 and 1.01, respectively, lower than that in other groups; the expression of Bax was 1.03, higher than that in other groups (p<0.05). The intervention with Ganoderma selenium rich protein can affect the proliferation and apoptosis of hepatoma cells, block cell cycle, inhibit invasion and migration, regulate the expression of Bcl-2, Bax and Caspase-3, which shows that Ganoderma selenium rich protein can regulate the proliferation, apoptosis, invasion and migration of hepatoma HepG2 cells, inhibit the development and diffusion of hepatoma HepG2 cells, and provide certain rationale for the clinical treatment of hepatoma.

国家自然科学基金地区基金项目（81760850）