本文研究维生素D改善肥胖大鼠脂代谢的作用。建立肥胖大鼠模型，正常组、模型组、高剂量组（2.40 μg/mL）、中剂量组（1.20 μg/mL）、低剂量组（0.60 μg/mL）、奥利司他组（0.10 mg/mL）。统计体重、体长、Lee’s指数、附睾脂肪垫质量、脂肪体重指数，检测总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C），观察大鼠脂肪细胞形态学，检测过氧化物酶体增殖物激活受体γ（PPAR-γ）、瘦素、线粒体解偶联蛋白（UCP）蛋白表达。高剂量组大鼠体重250.76 g、Lee’s指数2.52%、附睾脂肪垫质量5.05 g、脂肪体重指数1.41 g、TC 1.96 mmol/L、TG 0.83 mmol/L、LDL-C 1.05 mmol/L低于模型组大鼠，HDL-C 0.59 mmol/L高于模型组大鼠（p<0.05）。高剂量组大鼠PPAR-γ、瘦素蛋白表达低于模型组大鼠，高剂量组大鼠UCP蛋白表达高于模型组大鼠（p<0.05）。维生素D通过调控PPAR-γ、瘦素、UCP蛋白表达，改善大鼠脂代谢。

The effect of vitamin D on lipid metabolism in obese rats was investigated. Obesity rat model was established. The rats were divied into normal group, model group, high dose group (2.40 μg/mL), medium dose group (1.20 μg/mL), low dose group (0.60 μg/mL) and orlistat group (0.10 mg/mL). The body weight, body length, Lee's index, epididymal fat pad mass and fat body mass index were evaluated. Total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-C), low density lipoprotein (LDL-C) and the morphology of rat adipocytes were detected. Peroxisome proliferator-activated receptor gamma (PPAR-gamma), leptin and mitochondrial uncoupling protein (UCP) protein expression were also detected. The weight of rats, Lee's index, the weight of epididymal fat pad, fat body mass index, TC, TG, LDL-C and HDL-C in high dose group were 250.76 g, 2.52%, 5.05 g, 1.41 g, 1.96 mmol/L, 0.83 mmol/L, 1.05 mmol/L (lower than that in model group), and 0.59 mmol/L (higher than that in model group, p<0.05), respectively. The expression of PPAR-gamma and leptin in the high dose group was lower than that in the model group, and the expression of UCP in the high dose group was higher than that in the model group (p<0.05). Vitamin D can improve lipid metabolism in rats by regulating the expression of PPAR-gamma, leptin and UCP.

国家药典委项目（210）