研究长双歧杆菌DD98对高脂诱导的2型糖尿病及肥胖小鼠症状的缓解作用。糖尿病实验：采用C57BL/6N小鼠，将其随机分为正常对照组给予普通饲料；模型对照组，长双歧杆菌DD98干预组，空白培养基干预组给予高脂饲料，每组6只。4周后，链脲佐菌素（STZ）注射建模，给予受试物3周，实验结束后进行口服糖耐量实验和指标检测。与糖尿病模型组和空白培养基干预组相比，DD98干预组小鼠空腹血糖值（18.66±1.84 mmol/L）显著降低，并且DD98干预组小鼠葡萄糖耐量、血清和肝脏脂质水平（TG、TC、LDL-C）均得到显著改善。肥胖实验：采用C57BL/6N小鼠，将其随机分为正常对照组给予普通饲料；模型对照组，长双歧杆菌DD98干预组，空白培养基干预组给予高脂饲料，每组6只。同时给予受试物5周，进行指标检测。与高脂饲养组和空白培养基干预组小鼠相比，DD98干预组小鼠空腹体重值（20.89±2.51 g）、脏器指数、血清和肝脏脂质（TG、TC、LDL-C）水平均显著降低，肝脏脂质堆积情况得到明显改善。因此，长双歧杆菌DD98可以有效缓解2型糖尿病和肥胖症状，进一步改善由2型糖尿病和肥胖引起的血脂紊乱以及肥胖引起的轻度肝损伤。

The ameliorating effects of Bifidobacterium longum DD98 on high fat diet-induced type 2 diabetes mice and obesity in mice were investigated. Type 2 diabetes experiments: C57BL/6N mice were randomly divided into four groups (6 rmice per group): the normal chow group given a normal chow diet; the model control group, Bifidobacterium longum DD98 intervention group, and blank culture medium intervention group fed a high-fat diet. After four weeks, model mice with diabetes was established using streptozotocin (STZ). The mice were given the tested samples for 3 weeks, before glucose tolerance test and measurements of various indicators. Compared with the of the model group and the blank culture medium intervention group, the DD98 intervention group had a significantly decreased fasting blood-glucose (FBG) (18.66±1.84 mmol/L), along with significantly improved glucose tolerance and reduced serum/liver total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) levels. Obesity experiments: C57BL/6N mice were randomly divided into four groups (6 rmice per group): the normal chow group given a normal chow diet; the model control group, Bifidobacterium longum DD98 intervention group, blank culture medium intervention group with a high-fat diet. After five weeks of treatments, various indicators were measured. Compared with the high-fat diet group and the blank culture medium intervention group, the DD98 group had significantly lowered fasting body weight (20.89±2.51 g) and serum/liver TC, TG and LDL-C levels with significantly reduced fat accumulation in the liver. Therefore, Bifidobacterium longum DD98 could effectively ameliorate the symptoms of type 2 diabetes and obesity, and further alleviate dyslipidemia caused by type 2 diabetes and obesity, and liver injury caused by obesity.

国家自然科学基金项目（81573329）；上海市自然科学基金项目（16ZR1435400）