研究天麻提取物对大鼠抗缺氧能力的作用机制。通过建立大鼠常压耐缺氧实验模型，随机分为正常组、阳性对照组、低剂量天麻提取物组和高剂量天麻提取物组,统计大鼠的体重、生存时间、生存率。检测丙二醛（MDA）、超氧化物歧化酶(SOD)、总抗氧化能力（T-AOC）、过氧化氢酶（CAT）、乳酸脱氢酶（LDH）、乳酸（Lactate，LA）、谷胱甘肽（GSH）、谷胱甘肽还原酶（GR）水平、热休克蛋白70（HSp70）、内皮素-1（ET-1）、一氧化氮合酶（iNOS）、缺氧诱导因子-1α（HIF-1α）相关蛋白表达。研究结果表明：高剂量天麻提取物组大鼠生存时间提高了34.01%，LDH、LA、GSH、GR水平分别为8614.24 nmol/mL、2.01 nmol/mL、965.5 mg/mL、0.30 U/mL，HSp70蛋白表达为4.14，均显著高于正常组（p<0.05）；MDA、CAT水平分别为63.52 U/mL、23.24 U/mL，ET-1、iNOS、HIF-1α蛋白表达分别为1.96、2.10、0.28，均显著低于正常组（p<0.05）。说明天麻提取物能够有效的提高大鼠抗氧化能力，改善大鼠的氧化应激相关指标水平，从而提高大鼠机体的缺氧能力。

To study the mechanism of Gastrodia elata extract on anti-hypoxia ability in rats. The rats were randomly divided into normal group, positive control group, low-dose Gastrodia elata extract group and high-dose Gastrodia elata extract group. The body weight, survival time and survival rate of rats were investigated. Malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lactate dehydrogenase (LDH), lactate (LA), glutathione (GSH), glutathione reductase (GR), heat shock protein 70 (HSp70), endothelin-1 (ET-1), nitric oxide synthase (iNOS), the expression of related proteins for hypoxia inducible factor-1alpha (HIF-1a) were detected. The results showed that, compared with the normal group, the survival time of rats in the high dose Gastrodia elata extract group increased by 34.01%, the levels of LDH (8614.24 nmol/mL), LA (2.01 nmol/mL), GSH (965.5 mg/mL), GR (0.30 U/mL) increased (p<0.05), HSp70 (4.14), MDA (63.52 U/mL), CAT (23.24 U/mL), ET-1 (1.96), iNOS (2.10), HIF-1a (0.28) decreased (p<0.05). These results suggested that Gastrodia elata extract can effectively improve the antioxidant capacity of rats, the level of oxidative stress in rats, and thus improve the hypoxic capacity of rats.

内蒙古自治区教育科学研究“十三五”规划项目（NZJGH2018181）