研究葛根、藤茶、玉米低聚肽复合组方的解酒功能和保护肝脏活性。通过动物行为学实验，研究对比葛根、藤茶、玉米低聚肽单独使用及其联合使用的解酒效果，结果表明，与模型组比较，葛根、藤茶、玉米低聚肽复合组方能显著延长小鼠的醉酒时间至（55.14±20.97）min，缩短醒酒时间至（75.88±23.28）min（p<0.05），明显改善醉酒小鼠的行为学指标。通过建立小鼠慢性酒精性肝损伤模型，测定血清及肝组织生化指标，观察肝组织病理变化以及检测肝组织蛋白表达水平，研究复合组方的保肝作用，结果表明，与模型组比较，复合组方能够减轻酒精对小鼠肝细胞的损伤，显著使肝损伤模型小鼠血清中谷丙转氨酶(alanine aminotransferase，ALT)、谷草转氨酶(aspartate aminotransferase，AST)活性及肝组织中丙二醛（malondialdehyde，MDA）含量分别降低至（17.09±6.26）U/L，（21.41±6.80）U/L和（2.46±0.31）nmol/mg，肝组织中还原型谷胱甘肽（glutathione reduced，GSH）含量及超氧化物歧化酶(superoxide dismutase，SOD)活性分别增加至（8.80±2.14）nmol/mg和（241.99±35.60）U/mg（p<0.05），改善肝组织病理形态变化，下调肝组织中NF-κB、TNF-α表达。这表明复合组方具有解酒保肝作用，其作用机理可能与抗氧化活性、抑制炎症相关信号有关。

The anti-alcohol and hepatoprotective effect of a composite of pueraria, Ampelopsis grossedentata (AG) and corn oligopeptides (COP) were investigated. The anti-alcohol effects of pueraria, AG and COP used alone or in combination on drunk mice were evaluated through animal behavior experiments. The results showed that the pueraria-AG-COP composite (PAC composite) could significantly (p < 0.05) extend the waking time for drunkenness (up to 55.14±20.97 min) and shorten the period of hangover (to 75.88±23.28 min) while improving significantly the behavioral indices of the drunk mice, as compared with the model group. In the established chronic alcohol-induced liver injury mouse model, the hepatoprotective effect of PAC composite was investigated through measuring the biochemical indices of serum and liver tissues in mice, observing the pathological changes in hepatic tissues and examining the protein expression in liver tissues. Compared with the model group, the PAC composite could alleviate alcohol-induced liver damage in liver-injured mice: reducing significantly (p < 0.05) the activities of ALT and AST (to 17.09±6.26 and 21.41±6.80 U/L, respectively) in serum and the content of malondialdehyde (MDA; 2.46±0.31 nmol/mg) in liver tissues, whilst increasing significantly (p < 0.05) the content of glutathione (GSH, to 8.80±2.14 nmol/mg) and activity of superoxide dismutase (SOD; to 241.99±35.60 U/mg) in liver tissues. The PAC composite could also combat the pathological changes in liver tissues while down-regulating the protein expression of hepatic NF-κB and TNF-α in the liver-injured mice. All these results indicated that the PAC composite exhibited anti-alcohol and hepatoprotective effects through working mechanisms associated with its antioxidant activity and inhibition of the inflammatory-related signaling.