以紫色茄子皮为材料，期望分离纯化得到价格昂贵的高纯度飞燕草色素单体。利用光谱及质谱技术对紫色茄子皮的花青素苷进行了定性分析，鉴定出4种花青素苷，且均为连接不同糖配体的飞燕草色素苷。然后优化飞燕草色素的提取纯化条件，结果表明，提取剂为含1%盐酸的60%乙醇溶液、料液比为1:20时提取效果最佳。利用AB-8型大孔树脂初步纯化飞燕草色素苷，发现随着洗脱剂乙醇浓度的升高，得到的飞燕草色素苷溶液浓度先升高后降低，当乙醇浓度为30%时洗脱效果最好，此时洗脱液在530 nm下的紫外吸收值为0.49。运用高温酸水解的方法可获得飞燕草色素苷元，即飞燕草色素。对酸水解后获得的飞燕草色素苷元依次利用AB-8型大孔树脂和C18柱层析进行分离纯化，得到飞燕草色素单体，最后通过结晶沉淀的方法获得了纯度大于98%的飞燕草色素单体粉末。研究为分离纯化飞燕草色素提供了一种切实可行的方法。

In this work, the low price peel of purple eggplant was used as material to obtain expensive high purification delphinidin monomer. The anthocyanin in purple eggplant peel was qualitatively analyzed by spectrum and mass spectrometry, and 4delphindins were identified. Then the optimized conditions for extraction delphinidin were investigated. Result showed that optimal extraction solvent was 60% ethanol with 1% hydrochloric acid; ratio of solid to liquid was 1:20. AB-8 macroporous resin which eluted with ethanol was used for initial purification of anthocyanin, the concentration of 30% ethanol elute was the optimal, and the UV absorption value of 530 nm at this concentration was 0.49. Subsequently, the delphinidin glycoside was acid hydrolyzed, and delphinidin aglycone was purified by AB-8 macroporous resin and C-18 resin. Finally, delphinidin powder with purity more than 98% was obtained. It provides a practical method for separating and purifying delphinidin pigments.

国家自然科学基金项目（31470689；31570694）；安徽省自然科学基金项目（1708085QC51）；安徽农业大学稳定与引进人才项目（yj2017- 37）