以龙牙楤木为试验原料，采用盐析辅助酶法提取龙牙楤木皂苷，在单因素试验的基础上，采用响应面优化提取工艺并对比研究盐析辅助酶法、溶剂提取法制备的龙牙楤木皂苷抗氧化活性的区别。结果表明盐析辅助酶法最佳工艺为：酶解pH 5.405、酶解温度61.3 ℃、磷酸氢二钠添加量7.74%、酶解时间90 min、纤维素酶添加量1.5%，在此条件下龙牙楤木皂苷得率3.97%，该方法与溶剂提取法相比，可显著提高皂苷得率。抗氧化活性试验表明，盐析辅助酶法和溶剂提取法制备的龙牙楤木皂苷对DPPH自由基的IC50值依次为0.342 mg/mL、0.365 mg/mL，对超氧阴离子自由基的IC50值依次为1.220 mg/mL、1.432 mg/mL，总还原能力由强到弱为：Vc>盐析辅助酶法>溶剂提取法。本试验研究为龙牙楤木皂苷高效提取以及初步明确其抗氧化功能提供科学数据参考。

The aims of this study were to optimize the extraction process of the Aralia elata saponin and to evaluate its antioxidant capacity. A salting-out assisted enzymatically hydrolysis process was used to extract the saponin and optimized by response surface method. The product of solvent extraction was selected as control and the effects of this process on the antioxidant activities of the extract were investgated. The results showed that the optimum extraction conditions were as follows: pH 5.405, temperature 61.3 ℃, disodium phosphate concentration 7.74%, cellulase concentration 1.5% and duration 90 min. The productivity of Aralia elata saponin was 3.97% under these conditions. The productivity of this process significantly increased comparing with solvent extraction. Results from the antioxidant test showed that the IC50 values of products extracted by the optimized process and the solvent extraction process toward DPPH radicals were 0.342 mg/mL, 0.365 mg/mL, those toward superoxide radicals were 1.220 mg/mL, 1.432 mg/mL, respectively. The total antioxidant capacity superiority sequence was as follows: Vc > optimized process extracted product > solvent extracted product. This study provides scientific data for the efficient extraction and preliminary antioxidant function clarification of Aralia elata saponin.

“十三五”国家重点研发计划项目（2016YFC0500307-07）；哈尔滨市科技局科技创新人才研究专项基金（2017RAQXJ091）