测定一种传统中药米曲中总多酚、总黄酮及多糖含量，评估其抗氧化及免疫调节活性。采用超声波辅助提取法获得总多酚及总黄酮，通过水提醇沉法获得中药曲多糖。通过1,1-二苯基-2-三硝基苯肼(DPPH)自由基、羟自由基和超氧阴离子自由基的清除作用，评价中药曲多酚及黄酮的自由基清除能力。同时，测定中药曲多糖对小鼠脾细胞增殖的影响，进一步测定小鼠腹腔单核巨噬细胞细胞系(RAW 264.7)的吞噬能力和细胞因子分泌能力的促进作用。结果表明：中药曲中总多酚含量为33.54 mg/g，总黄酮含量为10.88 mg/g，多糖含量为74.98 mg/g。中药曲中多酚及黄酮具有优良的自由基清除活性。此外，中药曲多糖能直接促进脾细胞及免疫细胞的增殖。对于RAW 264.7细胞的吞噬能力及细胞因子分泌具有促进作用。传统中药米曲可作为一种保健品开发利用。

In order to evaluate the antioxidant and immunomodulatory activity of a traditional Chinese herbs-koji, the contents of total polyphenols, total flavonoids and polysaccharides were measured. Ultrasound-assisted extraction was used to obtain total polyphenols and total flavonoids. Polysaccharides in Chinese herbs-koji (HKP) were extrated by water extraction and alcohol precipitation. The antioxidant activity of polyphenols and flavonoids were evaluated by the use of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals, hydroxyl radicals, and superoxide anion radicals methods. The potency of HKP on the phagocytosis and cytokine secretion of RAW 264.7 cells was determined by commercial kit. Results showed that the content of total polyphenols in the Chinese herbs-koji was 33.54 mg/g, the total flavonoid content was 10.88 mg/g, and the polysaccharide content was 74.98 mg/g. The extracted polyphenols and flavonoids showed good free-radical scavenging capacity to DPPH radical, superoxide anion radical and hydroxyl radical. Furthermore, the corresponding half inhibitory concentration (IC50) of polyphenols is 18.87 μg/mL, 26.24 μg/mL and 33.31 μg/mL, and for flavonoids is 33.24 μg/mL, 38.97 μg/mL and 33.08 μg/mL. The HKP resulted in a significant increase of lymphocyte proliferation and could also enhance the effect of Con A-induced T lymphocyte proliferation and the stimulation index of LPS-induced B lymphocytes. In addition, the phagocytosis and cytokine secretion of RAW 264.7 cells can be promoted by HKP. The traditional Chinese herbs-koji can be developed and utilized as a health product.

四川省科技支撑计划项目(2016GZ0359)