禽蛋壳膜蛋白含量丰富，蛋白质约占蛋壳膜干重的90%，然而蛋壳膜在食品工业中通常被作为废弃物丢弃，造成了资源浪费和环境污染，因此高效地利用蛋壳膜资源是极为必要的。本研究使用亚硫酸钠辅助酶解法制备禽蛋壳膜抗氧化肽，研究了酶种类、酶添加量、亚硫酸钠浓度和酶解时间对禽蛋壳膜多肽抗氧化性和水解度的影响。结果表明，亚硫酸钠辅助下碱性蛋白酶酶解禽蛋壳膜，效果优于其他四种酶；亚硫酸钠辅助碱性蛋白酶酶解禽蛋壳膜的最佳条件是：碱性蛋白酶添加量12 U/mg，亚硫酸钠浓度40 mM，时间4 h。在最优工艺条件下，禽蛋壳膜多肽的总抗氧化能力为245.88 μg/mL，水解度为8.58%，表明禽蛋壳膜的亚硫酸钠辅助碱性蛋白酶酶解产物具有很好的体外抗氧化活性。SDS-PAGE表明禽蛋壳膜多肽的分子量主要在3.3~14.4 ku之间。

The eggshell membrane has abundant protein, which acounts for about 90% of the dry weight of the eggshell membrane. However, eggshell membranes are often discarded as waste in food industry, resulting in waste of resources and environmental pollution, thus, the efficient use of eggshell membranes is extremely necessary. In this study, the antioxidant peptides were prepared by sodium sulfite-assisted enzymatic hydrolysis. Effect of protease species, enzyme dosage, Sodium sulfite concentration and hydrolysis time on antioxidant activity and hydrolysis degree of eggshell membrane peptides was researched. The results showed that alkaline protease assisted by sodium sulfite was better than the other four proteases. At 12 U/mg alkaline protease, sodium sulfite concentration 40 mM, hydrolysis time 4 h, the degree of hydrolysis and total antioxidant activity of the hydrolysates were 8.58% and 245.88 μg/mL, respectively, indicating that the hydrolysates have good antioxidant activity in vitro. The results of SDS-PAGE and Tricine-SDS-PAGE showed that the molecular weight of the eggshell membrane polypeptide was mainly in the range of 3.3~14.4 ku.

“十三五”国家重点研发计划项目（2018YFD0400302）；国家自然科学基金资助项目（31471602）；蛋鸡产业技术体系岗位科学家项目（CARS-40-K24）