以罗非鱼鱼皮为原料，研究利用酶法制备胶原蛋白肽制品。测定了罗非鱼皮的基木组成成分，利用低温连续相变萃取技术进行脱脂处理；以水解度、水解产物分子量和感官评定为指标，研究蛋白酶种类对酶解的影响；以水解度为指标，研究酶解条件对水解度的影响，并采用响应面试验对鱼皮胶原肽制备工艺进行优化；用凝胶过滤色谱法测定酶解液分子质量分布。结果表明:采用碱性蛋白酶水解鱼皮效果最好，酶解的最佳工艺条件为：时间7 h、加酶量7%、温度60 ℃、pH值10.38，在此条件下碱性蛋白酶水解罗非鱼皮的水解度最高可达23.01%。由本法所得胶原肽是以分子质量180~1000 u之间的寡肽（约2~8个氨基酸残基）为主的物质，具有较好的生理活性，可作为功能因子广泛应用于保健食品、化妆品、医药等各个行业当中。

The collagen peptides from tilapia skin has been extracted by using an enzymatic method. The composition of tilapia skin was also analyzed. A novel continuous phase-transition extraction method at low temperature was used to remove fat from tilapia skin. The degree of hydrolysis, molecular weight and organoleptic investigation were used as indicators to study the effects of protease types on the hydrolysis. Response surface experiment was designed to optimize the preparation technology of the collagen peptide. GFC was used to determine the molecular weight distribution of hydrolysates. The results showed that alcalase was the best enzyme for the hydrolysis, and the optimal hydrolysis conditions were determined as follows: enzymolysis time 7 h, alcalase dosage 7%, temperature 60 ℃, and the pH 10.38. Under these conditions, the maximum hydrolysis degree was 23.01%. The peptides obtained is mainly composed of peptide with 2 to 8 amino acid residues, and has good physiological activity. It could be widely used as a functional factor in various industries, such as health food, cosmetics, medicine and other industries.

广东省省级科技计划项目（2013B051000071）