[关键词]
[摘要]
为研究老山芹不同溶剂提取物的活性功能,本研究老山芹为原料,比较了不同溶剂提取物的活性成分和抗氧化效果,以及不同溶剂提取物对α-葡萄糖苷酶和α-淀粉酶的影响,并对适宜条件下不同溶剂提取物对HepG2和panc-1细胞的影响进行研究。试验结果表明:随着浓度的增加两种酶抑制率而不断增大,并得出两种酶抑制率的IC50值,碱提取条件下α-葡萄糖苷酶和α-淀粉酶的IC50最小,分别为0.47 mg/mL、2.17 mg/mL。四种溶剂提取物中多糖含量最高的是碱提取,含量为58.09 mg/g;总酚含量最高的是醇提物,含量为11.48 mg/g;黄酮含量最高的是醇提物,含量为3.96 mg/g,并且四种提取物中醇提物的抗氧化活性最高,酸提物抗氧化活性最低。四种溶剂提取物在低浓度条件下,均能促进HepG2和panc-1细胞的增长,随着浓度的增加HepG2和panc-1细胞存活率均呈不同程度的下降,提取物在高浓度条件下对细胞有抑制作用。
[Key word]
[Abstract]
In order to study the active function of extracts from Heracleum Moellendorffii Hance by different solvents, the Heracleum Moellendorffii Hance was used as the raw material in this study. The active ingredients and antioxidant effects of different solvent extracts were compared. The effects of different extracts on the α-glucosidase and α-amylase were studied, and The effects of different solvent extracts on the HepG2 and panc-1 cells under the optimal conditions were also investigated. The results showed that with the increase of the concentration, the four extracts’ inhibitory rate of α-glucosidase and α-amylase also increased. The IC50 values of the two kinds of enzyme inhibitory rates were obtained, and the IC50 values of α-glucosidase and α-amylase in alkali extraction were the minimal value, which were 0.47 mg/mL and 2.17 mg/mL, respectively. The highest content of polysaccharides in the four solvent extracts was alkali extraction, the content was 58.09 mg/g; The highest content of total phenols was ethanol extract, the content was 11.48 mg/g; The highest content of flavonoids was ethanol extract, the content was 3.96 mg/g. The alcohol extract of the four extracts had the highest antioxidant activity, and the acid extract had the lowest antioxidant activity. All the four extracts could promote the proliferation of HepG2 and panc-1 cells at low concentrations. With the increase of concentration, the survival rates of HepG2 and panc-1 cells decreased in different degrees. Extracts had inhibitory effect on cells under high concentration.
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[基金项目]
十三五国家重点研发计划项目(2016YFC0500307-07)