依据GB/T 15000.3-2008标准样品的工作导则，研制和厚朴酚标样样品。以厚朴药材的提取物为原料，采用高速逆流色谱法一步分离制备和厚朴酚单体化合物，通过紫外光谱、红外光谱、质谱、1H和13C核磁共振等技术手段进行化合物的结构表征，同时进行了薄层色谱、液质联用和热重分析纯度和水分含量，建立了和厚朴的高效液相色谱分析检测技术。和厚朴酚样品进行了均匀性检验、稳定性检验和8家实验室联合定值。结果表明，和厚朴酚样品具有良好的均匀性，在0~4 ℃储存条件下48个月含量稳定。将定值结果分析统计，确定其标准值为99.72%，置信度95%的不确定度为0.22%。成功研制出了和厚朴酚国家标准样品，达到了GB/T 15000.3-2008规定的技术要求，样品具有溯源性，可用于和厚朴酚及相关产品的质量控制和检测方法评定。

The certified reference material (CRM) of honokiol was develpoed according to the technical requirements of CRM General and Statistical Principle for Certification (GB/T 15000.3-2008). Honokiol was separated and prepared by high-speed counter-current chromatography with crude extracts of Magnolia officinalis Rehd. et Wils. as material, and their structures were confirmed by UV, IR, MS, 1H and 13C NMR. Simultaneously, the purity and moisture content of Honokiol was analyzed by thin layer chromatography, LC-MS, and thermo-gravimetric to establish HPLC analysis technology. Honokiol was divided into bottles for homogeneity test, stability test, and cooperative certification by 8 laboratories. The results indicated that the homogeneity of honokiol samples were excellent, with good stability within 48 months at 0-4oC. The certified value of the reference material of honokiol was 99.72% with the expanded uncertainty of 0.22% in confidence coefficient of 95%. The reference material of honokiol was successfully developed, which reached the technical requirement of GB/T 15000.3-2008 and could be traceable. The CRM can be used in the content determination and testing method validation of honokiol related products.

山东省重大科技创新工程（2017CXGC1308）；山东省重点研发计划（2017GSF216002）；山东省泰山学者岗位专家支持项目；山东省科学院先导项目