采用ELISA法测定比较不同剂量益生菌复合制剂和脾虚证经典方剂四君子汤对番泻叶水煎液诱导的脾虚证大鼠血清胃肠激素和免疫因子水平的影响，考察其对脾虚证大鼠的调节作用。结果表明，益生菌复合制剂和四君子汤均能显著降低脾虚证大鼠血清血管活性肠肽（Vasoactive intestinal peptide，VIP）及丙二醛（Malondialdehyde，MDA）水平（p<0.05），且均可显著升高白介素-2（Interleukin-2，IL-2）水平（p<0.05）。此外，益生菌复合制剂可显著降低脾虚证大鼠血清胆囊收缩素（Cholecystokinin，CCK）、P物质（Substance P，SP）及白介素-4（Interleukin-4，IL-4）水平（p<0.05）；四君子汤能极显著降低脾虚证大鼠血清胃泌素（Gastrin，GAS）及胃动素（Motilin，MTL）水平（p<0.01）。益生菌复合制剂能够调节脾虚证大鼠胃肠激素水平，改善其免疫功能。与传统脾虚证治疗方剂四君子汤相比，益生菌复合制剂与四君子汤所调节的胃肠激素和免疫因子指标不同，提示二者治疗脾虚证的作用机制可能不一。

ELISA kit was used to investigate the regulatory effects of different doses of composite probiotics and Sinijunzi Decoction on the levels of serum gastrointestinal hormones and immune factors in spleen deficiency syndrome rats induced by Folium Sennae. The results showed that both composite probiotics and the Sijunzi Decoction could significantly reduce the serum levels of vasoactive intestinal peptide (VIP) and malondialdehyde (MDA) (p < 0.05), while increase the interleukin-2 (IL-2) level in spleen deficiency syndrome rats (p < 0.05). The composite probiotics could also significantly reduce the levels of serum cholecystokinin (CCK), substance P (SP) and interleukin-4 (IL-4) in spleen deficiency rats (p < 0.05), while the Sijunzi Decoction had no significant effect. In addition, the Sijunzi Decoction could significantly decrease the content of serum gastrin (GAS) and motilin (MTL) in spleen deficiency rats (p < 0.01). The results showed that composite probiotics could regulate the levels of gastrointestinal hormone, as well as correcting immune dysfunction of spleen deficiency rats. Compared with the traditional prescription (Sijizitang decoction) for the treatment of spleen deficiency syndrome, the probiotics and Sijunzi decoction had different indicators of gastrointestinal hormones and immune factors, suggesting that the mechanisms of composite probiotics and the Sijunzi Decoction treating on spleen deficiency syndrome may be different.

国家自然科学基金资助项目（30973962）