[关键词]
[摘要]
以新疆赛买提杏为试验原料,选用0.9 mg/L的24-表油菜素内酯(24-epibrassionolide,EBR)在0.05 MPa的压强下对杏果实进行减压渗透处理,以蒸馏水处理作为对照,将处理后的果实取出晾干后在温度为4 ℃、RH 90%~95%的冷库中贮藏。定期测定杏果实的冷害发病率和冷害指数及过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、超氧阴离子自由基(O2-·)的产生速率和过氧化氢(H2O2)的含量。结果表明:浓度为0.9 mg/L的24-表油菜素内酯能有效提高杏果实SOD、APX的活性,减缓杏果实冷藏期间POD和CAT活性的下降,抑制H2O2含量和O2-·产生速率的增长,显著降低杏果实冷害发病率和冷害指数。说明24-表油菜素内酯处理减轻杏果实冷害与防止果实冷藏期间的氧化伤害,清除自由基的积累密切相关。
[Key word]
[Abstract]
Saimaiti apricots sampled from Xinjiang were infiltrated by 0.9 mg/L 24-epibrassinolide (EBR) under the pressure of 0.05 MPa, and the distilled water treatment was used as a control. The treated fruits were taken out, air-dried and stored in a cold room at 4℃ and relative humidity of 90%-95%. Incidence of chilling injury and chilling injury index, the activities of catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD),peroxidase (POD) and superoxide anion (O2-) and the content ofhydrogen peroxide (H2O2) of apricot fruits were determined regularly. The results showed that 0.9 mg/L 24-epibrassionolide treatment could increase the activities of SOD and APX, and decreased the activities of POD and CAT in apricot fruit during cold storage. It also inhibited the content of H2O2 and the increase of generation rate of O2-, and significantly decreased the incidence of chilling injury and chilling injury index of apricot fruit. Results indicated that the 24-epibrassionolide treatment was related to the reduction of chilling injury, prevention of oxidative damage and the accumulation of free radical scavenging during apricot preservation.
[中图分类号]
[基金项目]
国家自然科学基金资助项目(31460414);公益性行业(农业)科研专项(201303075);南京农业大学-新疆农业大学联合基金项目(KYYJ201605)