寻找食源性致病沙门氏菌（Salmonella）血清型生物标志物，是研发食源性致病微生物鉴定及监控技术的基础。本文基于代谢组学技术提取5种沙门氏菌血清型8株标准菌株胞内代谢产物，对各菌株进行胞内代谢轮廓分析，并构建指纹图谱和PCA分析。研究发现，各血清型之间的产物在种类和丰度上具有较大差异，其中肠炎沙门氏菌血清型的特异性产物为硫基乙酸和α-羟基-戊二酸，伤寒沙门氏菌血清型特有产物为N-乙酰基-天冬氨酸、2(1H)-嘧啶酮和L-苏糖酸，鼠伤寒沙门氏菌血清型特有产物为肉豆蔻酸甲酯和甘油醚葡萄糖苷，猪霍乱沙门氏菌血清型特有产物1,4-萘醌，亚利桑那沙门菌血清型特有产物为3-吡啶甲酸三甲酯；通过聚类分析和PCA分析可以明显区分这5种沙门氏菌血清型。因此以上特异性产物有望作为各自血清型的潜在生物标志物。

Identifying the characteristic metabolites of foodborne pathogenic Salmonella serotypes is the basis for the research and development of an identification and monitoring technique for foodborne pathogenic microorganisms. In this study, intracellular metabolites were extracted from five different Salmonella serotypes comprising eight standard strains by using metabolomics technology, and intracellular metabolic profile analysis was conducted for each strain. Additionally, fingerprints were constructed and principal component analysis (PCA) was performed. It was found that the metabolites of each serotype had large differences in terms of type and abundance, and some potential characteristic metabolites were found. For example, mercaptoacetic acid and α-hydroxypentanedioic acid were biomarkers of the S. enteritidis serotype; N-acetyl aspartic acid, 2(1H)-pyrimidone, and L-threonic acid were only produced by the S. typhi serotype; tetradecanoic acid methyl ester and glyceryl ether-glucoside were released only by S. typhimurium serotype; 1,4-naphthoquinone was only produced by S. choleraesuis serotype, and 3-pyridinecarboxylic acid trimethylsilyl ester was unique to S. arizonae serotype. The five Salmonella serotypes could be clearly distinguished by cluster analysis and PCA. As a result, the above specific products could be used as potential biomarkers of Salmonella serotypes.

广东省科技计划项目（2014A040401055、2015B020230006）