[关键词]
[摘要]
家蚕蛹是蚕桑产业中的重要副产物资源,因营养丰富、蛋白质含量高、富含不饱和脂肪酸等优点,常用作人们的休闲食品,近年来,随着以蚕蛹为原料的多元化产品开发研究的不断发展,蚕蛹资源的利用越来越成为增加蚕桑产业效益的重要途径,但因食用或饲用家蚕蛹而引发的过敏症状较为严重,制约了其在食品和饲料中的应用。已有报道家蚕蛹中的糖水解酶18家族几丁质酶(BmChi)是一种重要变应原物质,但至今尚无其三维结构数据,本文通过生物信息学软件分析,对家蚕蛹糖水解酶18家族几丁质酶的同源性、二级结构、亲疏水性、可塑性、表面可及性和抗原性等进行了综合分析,确定其抗原表位位点,为深入开展该变应原的免疫学特性研究打下基础,并为家蚕蛹蛋白的脱敏研究提供理论基础。
[Key word]
[Abstract]
Domestic silkworm (Bombyx mori) pupa is an important byproduct of the sericulture industry and is commonly consumed as a snack owing to its nutritional properties and high content of proteins and unsaturated fatty acids. Recent progress in the study and development of diversified products prepared from silkworm pupa has boosted the revenue obtained from the sericulture industry. However, the severe allergy symptoms resulting from consuming silkworm pupa have limited its applications in the food and feed industries. It has been reported that the glycoside hydrolase family 18 chitinase (BmChi) from domestic silkworm (Bombyx mori) pupa is an important food allergen; however, no 3-dimentional structure studies have been conducted on the allergen BmChi. In this study, several bioinformatics software tools were used to comprehensively analyze the homology, secondary structure, hydrophilicity and hydrophobicity, molecular flexibility, antigenicity, and surface accessibility of BmChi, and subsequently, the B-cell epitope region was predicted. The results of this study can provide the foundation for an in-depth study on the immunological characteristics of BmChi and help developing methods for desensitization to silkworm pupa.
[中图分类号]
[基金项目]
国家自然科学基金项目(31501421);国家公益性农业行业科技专项(201403049);广东省教育部产学研专项资金项目(2013B090600060)