采用柠檬酸钠还原法制备了银溶胶以及三种不同粒径大小的金溶胶作为拉曼表面增强剂，通过紫外-可见吸收光谱及透射电镜(TEM)比较其尺寸、粒度、分散性及稳定性，后选取盐酸氯丙那林、硫酸特布他林和盐酸班布特罗三种β受体激动剂类药物作为检测对象，观察拉曼信号增强效果。结果表明，金、银溶胶对β受体分子的表面增强拉曼信号增强信号基本上没有干扰。金溶胶的纳米粒子大小对于吸附能力有一定程度的影响，其颗粒大小更易于通过加入柠檬酸钠的方式进行调节，而银纳米溶胶颗粒粒径较不易控制。金溶胶能较长时间保持稳定，而银溶胶较易发生沉淀，但银纳米颗粒与目标分子结合后能产生更为强烈的共振现象，与金溶胶相比具有更强的表面增强活性，在拉曼信号增强目标分子时效果更好。

The sodium citrate-reduction method was adopted to prepare gold nanoparticle sols (three sizes) and one silver nanoparticle sol as Raman surface enhancers, and their size, granularity, dispersity, and stability were determined by ultraviolet–visible spectroscopy (UV-Vis) and transmission electron microscopy (TEM). Raman signal enhancement effect was analyzed by testing three β agonists: clorprenaline hydrochloride, terbutaline sulfate, and bambuterol hydrochloride. The results demonstrated that gold and silver nanoparticle sols showed no interference in the enhanced Raman signals from the surface-enhanced Raman scattering of the three β agonists. The size of the gold nanoparticles had an impact on sol absorptivity, which could be adjusted easily by adding sodium citrate, while the size of the silver nanoparticles was not easy to control. The gold sol could remain stable for a long period, while precipitation could easily occur in the silver sol. However, after binding with the target molecules, the silver nanoparticles could produce stronger resonance phenomena and exhibit a stronger surface enhancement effect than the gold nanoparticles. Therefore, it was more effective on the Raman signal enhancement of target molecules.

中国食品药品检定研究院“中青年发展研究基金”项目(2013WC3)；“广西自然科学青年基金”项目(2014GXNSFBA118059)