本文采用组织分离法从片栗叶部分离获得一株内生真菌，通过28s rDNA序列测定和系统发育树分析对此菌株进行了鉴定。用PD培养基和YM培养基分别发酵培养此菌株，其次级代谢产物通过高效液相色谱（HPLC）和质谱（MS）进行了分析。在YM培养基条件下对此菌株大量培养，利用硅胶层析等手段对其次级代谢产物进行分离纯化，采用MTT法测定了次级代谢产物对癌细胞的毒性。结果表明，从片栗叶部分离得到的内生真菌鉴定为Eupenicillium sp. NF2_PD013。经HPLC和MS分析表明，此内生真菌的次级代谢产物在YM培养基条件下比在PD培养基条件下丰富且产量大。从YM培养基条件下的次级代谢产物中分离得到4个化合物，分别为geodin，dihydrogeodin，3’-chlorotrypacidin和monochlorosulochrin，4个化合物均含有氯元素。化合物geodin和3’-chlorotrypacidin对乳腺癌细胞MCF-7和肝癌细胞HepG2都有明显的增殖抑制作用。

An endophytic fungus was isolated from Erythronium japonicum Decne. Using a tissue separation method, and this fungus was identified by 28S rDNA sequencing and phylogenetic analysis. The strains were cultured in potato dextrose (PD) and yeast and malt extract (YM) media, and their secondary metabolites were analyzed using high-performance liquid chromatography (HPLC) and mass spectrometry (MS). This strain was cultivated on a large scale in YM medium, while the secondary metabolites were separated and purified using silica gel chromatography. The cytotoxicity of the compounds isolated from secondary metabolites was determined by MTT assay. The endophytic fungus from Erythronium japonicum Decne. was identified as Eupenicillium sp. NF2_PD013. HPLC and MS analyses indicated that there were more secondary metabolites of this endophytic fungus in YM medium with a higher yield than those in PD medium. The four chlorine-containing compounds obtained from the secondary metabolites in YM medium were identified as geodin, dihydrogeodin, 3’-chlorotrypacidin, and monochlorosulochrin. Geodin and 3’-chlorotrypacidin showed significant inhibitory effects on the proliferation of breast cancer cells MCF-7 and hepatocellular carcinoma cells HepG2.

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