为了建立准确、高效的玉米黄质及其异构体的检测方法，通过比较不同流动相、流动相比例和洗脱时间对玉米黄质碘催化-光异构化样品分离效果，采用光谱、色谱、质谱多种方法对玉米黄质及其异构体进行定性定量分析。结果表明：玉米黄质及其异构体最佳的检测条件是流动相A：甲醇、MTBE和水，流动相B：甲醇和MTBE，流动相比例A为80/15/5，B为1/10，洗脱时间为24 min；通过对各个峰最大吸收波长、Q值、质谱离子碎片和与相关报道比对，在玉米黄质碘催化-光异构化样品中鉴定出了15/15’-顺式、13/13’-顺式和9/9’-顺式三种玉米黄质顺式异构体；全反式玉米黄质在5~250 ng范围内峰面积与进样量呈良好线性关系。该方法具有重现性好、准确度高、检测快速等优势，可用于玉米黄质及其异构体的检测

In order to establish a method to accurately and effectively detect zeaxanthin and its stereoisomers, the zeaxanthin products formed by iodine-catalyzed photoisomerization were separated using different mobile phases, ratios of the mobile phase, and elution times. The qualitative and quantitative analyses of zeaxanthin and its stereoisomers were conducted using spectroscopy, chromatography and mass spectrometry. The results indicated that the optimum conditions for the detection of zeaxanthin and its stereoisomers were as follows: mobile phase A: methanol, methyl tert-butyl ether (MTBE), and water (ratio, 80/15/5); mobile phase B: methanol and MTBE (ratio, 1/10); elution time: 24 minutes. In the zeaxanthin products from the iodine-catalyzed photoisomerization, 15,15′-, 13,13′-, and 9,9′-mono-cis-zeaxanthin isomers were identified by comparing the maximum absorption wavelength for each peak, Q value, and ion fragments in mass spectra with those in related reports. Peak area and injection volume of all-trans-zeaxanthin showed a good linear relationship in the range of 5–250 ng. The proposed method showed advantages including good reproducibility, high accuracy, and rapid detection, which is suitable for the detection of zeaxanthin and its stereoisomers.

江苏省农业科技自主创新资金项目（CX（14）2055）