本文首先采用高碘酸氧化、Smith降解、气相色谱分析、甲基化、气质联用等方法，对海蚬多糖（CPS）纯化组分（f2、f3）结构进行解析，研究表明，f2、f3具有不同的糖链结构，f2主链由(1→3)-葡萄糖、(1→4)-葡萄糖构成，支链为(1→2)-葡萄糖、(1→6)-葡萄糖。f3主链为(1→3)-葡萄糖、(1→3)-半乳糖，支链为(1→6)-葡萄糖。其次，采用四氯化碳（CCl4）诱导小鼠肝损伤模型对CPS护肝活性进行研究，结果表明，CPS组能够显著提高小鼠肝超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性（P < 0.05），降低小鼠血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）及肝丙二醛（MDA）水平（P < 0.05），表明CPS对CCl4诱导的肝损伤小鼠具有保护作用。CPS的护肝效果可能与其提高小鼠自身抗氧化能力以及抑制脂质过氧化有关。

The structure of purified components (f2 and f3) of Corbiculidae polysaccharide (CPS) was studied using periodate oxidation, Smith degradation, gas chromatography (GC), methylation, and GC-mass spectrometry (MS) techniques. The results showed that f2 and f3 had different sugar chain structures. The main chain of f2 was composed of a α-D-(1→3) linked glucose (Glc) and α-D-(1→4) linked Glc, with the branch being composed of α-D-(1→2) linked Glc and α-D-(1→6) linked Glc. The main chain of f3 contained α-D-(1→3) linked Glc and α-D-(1→3) linked galactose, while the branch contained α-D-(1→6) linked Glc. The hepatoprotective activity of CPS was evaluated using a mouse model of carbon tetrachloride (CCl4)-induced liver injury. The results showed that oral administration of CPS significantly reduced the elevated serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and liver malondialdehyde (MDA) level (P < 0.05) and significantly increased the activities of liver superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P < 0.05). This suggests that CPS exhibits potent hepatoprotective effects on CCl4-induced liver damage in mice, which may be related to the enhancement of antioxidant capacity and inhibition of lipid peroxidation in mice.

江苏省高校自然科学研究项目（13KJB550004）