[关键词]
[摘要]
本文研究了富士苹果渣中总三萜体外抗氧化活性及其体内对小鼠CCl4急性肝损伤的保护作用。采用超声波辅助提取、大孔树脂纯化得到苹果渣总三萜,以抗坏血酸(Vc)为对照,测定苹果渣总三萜清除DPPH?、ABTS+?、?OH的能力,评价其体外抗氧化活性;建立CCl4急性肝损伤模型,生化法测定小鼠血清中ALT、AST的活性,肝组织匀浆中SOD、GSH-Px的活性及MDA、IL-6的含量。结果表明:苹果渣总三萜具有一定的体外抗氧化能力,但不及Vc。在体内实验中与模型组相比,血清中ALT、AST活性显著下降,最高达48%以上;肝组织中SOD、GSH-Px活性显著升高,最高达69%;MDA、IL-6含量显著降低,最高达43%。苹果渣总三萜对CCl4急性肝损伤有明显保护作用,其机制可能与清除体内自由基,调节血清酶、抗氧化物酶的活力和含量有关。
[Key word]
[Abstract]
The in vitro antioxidant activity and in vivo protective effect of total triterpenoids in Fuji apple pomace (TTAP) on CCl4-induced acute liver injury in mice wereinvestigated. TTAP was isolated and purified by ultrasonic-assisted extraction and macroporous resin column chromatography, respectively. Using ascorbic acid (Vc) as control, the 2,2-diphenyl-1-picryl-hydrazyl (DPPH?); 2,2’-azino-di-(3- ethylbenzthiazoline-6-sulphonic acid (ABTS+?), and hydroxyl (?OH) scavenging abilities of TTAP were measured to evaluate its antioxidant activity. A CCl4-induced acute liver injury mouse model was established. Subsequently, aspartate transaminase (ALT) and aspartate transaminase (AST) activities in serum as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, malondialdehyde (MDA) content and interleukin-6 (IL-6) content in mouse liver homogenates were measured by biochemical assays. TTAP showed lesser extent of in vitro antioxidant ability compared with Vc. The in vivo study showed that ALT and AST activities in serum declined significantly and the largest decline was more than 48% when compared with the model group. Additionally, SOD and GSH-Px activities in liver homogenates significantly increased, with the highest increase of 69%. MDA and IL-6 contents declined significantly, with the largest decline of 43%. TTAP thus exhibited significant protective effect on CCl4-induced liver injury mouse model and the underlying mechanism may be related to in vivo free-radical scavenging and regulation of serum enzymes, as well as activity and content of antioxidant enzymes.
[中图分类号]
[基金项目]
农业部现代技术体系苹果专项资助项目(MATS)(Z225020701);陕西省农业科技创新与攻关项目(2015NY004)