本文分别以蔗渣和棉籽壳为基质对灵芝进行栽培，对比两种培养基中的灵芝生长性状、相关酶活变化规律和灵芝子实体的营养成分。结果表明：以蔗渣为基质栽培的灵芝菌丝生长速度（1.82±0.54 cm/d）较快、生物学效率（23.71±1.49%）较高，生长周期比棉籽壳培养基的缩短1天。灵芝在以蔗渣和棉籽壳为基质的栽培过程中，羧甲基纤维素酶、木聚糖酶和漆酶活性变化规律基本一致，两种培养基的灵芝三种酶活性在菌丝生长期和子实体生长期各有一个峰值。以蔗渣为基质的灵芝羧甲基纤维素酶和木聚糖酶的活性总体较高，漆酶活性总体低于棉籽壳为基质的灵芝。蔗渣为基质的灵芝子实体的粗蛋白（22.04±0.07%）、粗脂肪（2.73±0.03%）、多糖（4.15±0.03%）和粗纤维含量（34.19±3.15%）较高；而灰分含量（3.94±1.08%）、氨基酸总量（10.40%）、必需氨基酸量（4.25%）、必需氨基酸占氨基酸总量的比值（40.88%）均低于棉籽壳为基质的灵芝子实体。

In this study, Ganoderma lucidum was cultured using bagasse and cottonseed hull as substrates, and the growth characteristics, enzyme activity, and nutrients in the fruiting body were compared. The results showed that G. lucidum cultivated on bagasse exhibited faster mycelium growth (1.82 ± 0.54 cm/d), higher biological efficiency (23.71 ± 1.49%), and a shorter growth cycle (less by a day) than G. lucidum cultivated on cottonseed hull. During cultivation on either substrate, the trend of changes in the enzyme activity of carboxymethyl cellulase (CMCase), xylanase, and laccase in two culture media were consistent. A single peak value for each of the three enzyme activities was obtained for G. lucidum cultivated on the two substrates in the mycelium and fruiting body stages. G. lucidum cultivated on bagasse showed higher CMCase and xylanase activities, but lower laccase activity when than G. lucidum cultivated on cottonseed hull. Compared with the fruiting body of G. lucidum cultivated on cottonseed hull, the fruiting body of G. lucidum cultivated on bagasse substrate showed higher content of crude protein (22.04 ± 0.07%), crude fat (2.73 ± 0.03%), polysaccharide (4.15 ± 0.03%), crude fiber (34.19 ± 3.15%), lower content of amino acids (10.40%), essential amino acids (4.25%), ash (3.94 ± 1.08%), and a lower ratio of essential amino acid to total amino acid (40.88%).

国家级大学生创新创业训练计划（201210674020）；云南省科技厅应用基础研究计划项目（2011FZ046）